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counterstaining nuclei with dapi  (Beyotime)


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    Structured Review

    Beyotime counterstaining nuclei with dapi
    Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and <t>DAPI-stained</t> nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Counterstaining Nuclei With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/counterstaining+nuclei+with+dapi/DAPI/pmc12858359-53-15-21
    Average 99 stars, based on 32249 article reviews
    counterstaining nuclei with dapi - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Bioinspired lipid droplets nanoplatform for periodontitis therapy: Integrated antibacterial, mitochondrial repair, and immunomodulatory functions"

    Article Title: Bioinspired lipid droplets nanoplatform for periodontitis therapy: Integrated antibacterial, mitochondrial repair, and immunomodulatory functions

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2026.102808

    Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and DAPI-stained nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and DAPI-stained nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Immunofluorescence, Fluorescence, Staining, Western Blot, Biomarker Discovery, Expressing

    Related Articles

    Microscopy:

    Article Title: Parthanatos Involves in the Damage of Hippocampal Neurons Following Epileptic Seizure: in Vivo and Vitro Study
    Article Snippet: After that, the cells were incubated with urochromeconjugated secondary antibody against mouse (1: 200, Abcam, Cambridge, UK, USA) or rabbit (1: 200, Abcam, Cambridge, UK, USA) for 1 h at room temperature. .. After counterstaining nuclei with DAPI (Beyotime Biotec, Jiangsu, China) for 5 min, the cells were visualized under uorescence microscope (Olympus IX71, Tokyo, Japan). .. 2.9 Mitochondrial membrane potential (JC-1) assay HT22 cells treated with glutamate at indicated doses for different time and pretreated 1 h with 20 μmol/L PJ34 or knocking down of PARP-1 with siRNA overnight followed by glutamate incubation, were collected and stained with JC-1 (Beyotime Biotech, Nanjing, China) followed the methods described previously by Ma et al [21].

    Article Title: AR-A014418, a glycogen synthase kinase-3β inhibitor, mitigates lipopolysaccharide-induced inflammation in rat dental pulp stem cells via NLR family pyrin domain containing 3 inflammasome impairment
    Article Snippet: .. Afterward, rDPSCs were cultivated with the primary antibody against NLRP3 (PA5-79740, ThermoFisher) overnight at 4 °C, followed by incubation with Dylight 488-labeled secondary antibody (35,552, ThermoFisher) for 1 h. Finally, the stained cells were observed using confocal fluorescence microscopy (Leica microsystems, IL, USA) after counterstaining nuclei with DAPI (Beyotime) for 10 min. ..

    Staining:

    Article Title: Bioinspired Lipid Droplets Nanoplatform for Periodontitis Therapy: Integrated Antibacterial, Mitochondrial Repair, and Immunomodulatory Functions
    Article Snippet: 1 μg mL− 1 LPS (Sigma, USA) was subsequently added for 18h to recruit endogenous CRAMP onto LDs surfaces through innate immune activation. .. Synthesis conditions were screened by staining LDs with BODIPY 493/503 (5 μM, MedChemExpress, USA) and counterstaining nuclei with DAPI (7 μM, Beyotime, China). .. Three-dimensional imaging via confocal fluorescence microscopy (Nikon TI2+A1 R, Japan) was performed to measure the number and fluorescent area of intracellular LDs at three time points: following 12 h of OA treatment, following 12 h of OA treatment+4 h of GA incubation, and following 12 h of OA treatment+4 h of GA incubation+18 h of LPS stimulation.

    Article Title: AR-A014418, a glycogen synthase kinase-3β inhibitor, mitigates lipopolysaccharide-induced inflammation in rat dental pulp stem cells via NLR family pyrin domain containing 3 inflammasome impairment
    Article Snippet: .. Afterward, rDPSCs were cultivated with the primary antibody against NLRP3 (PA5-79740, ThermoFisher) overnight at 4 °C, followed by incubation with Dylight 488-labeled secondary antibody (35,552, ThermoFisher) for 1 h. Finally, the stained cells were observed using confocal fluorescence microscopy (Leica microsystems, IL, USA) after counterstaining nuclei with DAPI (Beyotime) for 10 min. ..

    Article Title: Bioinspired lipid droplets nanoplatform for periodontitis therapy: Integrated antibacterial, mitochondrial repair, and immunomodulatory functions
    Article Snippet: 1 μg mL −1 LPS (Sigma, USA) was subsequently added for 18h to recruit endogenous CRAMP onto LDs surfaces through innate immune activation. .. Synthesis conditions were screened by staining LDs with BODIPY 493/503 (5 μM, MedChemExpress, USA) and counterstaining nuclei with DAPI (7 μM, Beyotime, China). .. Three-dimensional imaging via confocal fluorescence microscopy (Nikon TI2+A1 R, Japan) was performed to measure the number and fluorescent area of intracellular LDs at three time points: following 12 h of OA treatment, following 12 h of OA treatment+4 h of GA incubation, and following 12 h of OA treatment+4 h of GA incubation+18 h of LPS stimulation.

    Incubation:

    Article Title: AR-A014418, a glycogen synthase kinase-3β inhibitor, mitigates lipopolysaccharide-induced inflammation in rat dental pulp stem cells via NLR family pyrin domain containing 3 inflammasome impairment
    Article Snippet: .. Afterward, rDPSCs were cultivated with the primary antibody against NLRP3 (PA5-79740, ThermoFisher) overnight at 4 °C, followed by incubation with Dylight 488-labeled secondary antibody (35,552, ThermoFisher) for 1 h. Finally, the stained cells were observed using confocal fluorescence microscopy (Leica microsystems, IL, USA) after counterstaining nuclei with DAPI (Beyotime) for 10 min. ..

    Article Title: miR-27b Represses Migration of Mouse MSCs to Burned Margins and Prolongs Wound Repair through Silencing SDF-1a
    Article Snippet: .. On the third day, the slides were washed to clear unbound DNA sequences, followed by incubated in primary monoclonal Pan-CK antibody (1∶50) at 37°C for 5 min and in secondary antibody (1∶200) labeled with FITC (Invitrogen, USA) at 37°C for 90 min, and counterstaining nuclei with DAPI (Beyotime, China) . .. The sections were analyzed by laser scanning confocal microscopy in ten random fields (Leica, Germany).

    Fluorescence:

    Article Title: AR-A014418, a glycogen synthase kinase-3β inhibitor, mitigates lipopolysaccharide-induced inflammation in rat dental pulp stem cells via NLR family pyrin domain containing 3 inflammasome impairment
    Article Snippet: .. Afterward, rDPSCs were cultivated with the primary antibody against NLRP3 (PA5-79740, ThermoFisher) overnight at 4 °C, followed by incubation with Dylight 488-labeled secondary antibody (35,552, ThermoFisher) for 1 h. Finally, the stained cells were observed using confocal fluorescence microscopy (Leica microsystems, IL, USA) after counterstaining nuclei with DAPI (Beyotime) for 10 min. ..

    Labeling:

    Article Title: miR-27b Represses Migration of Mouse MSCs to Burned Margins and Prolongs Wound Repair through Silencing SDF-1a
    Article Snippet: .. On the third day, the slides were washed to clear unbound DNA sequences, followed by incubated in primary monoclonal Pan-CK antibody (1∶50) at 37°C for 5 min and in secondary antibody (1∶200) labeled with FITC (Invitrogen, USA) at 37°C for 90 min, and counterstaining nuclei with DAPI (Beyotime, China) . .. The sections were analyzed by laser scanning confocal microscopy in ten random fields (Leica, Germany).



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    Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and <t>DAPI-stained</t> nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and <t>DAPI-stained</t> nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and DAPI-stained nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Bioinspired lipid droplets nanoplatform for periodontitis therapy: Integrated antibacterial, mitochondrial repair, and immunomodulatory functions

    doi: 10.1016/j.mtbio.2026.102808

    Figure Lengend Snippet: Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and DAPI-stained nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Synthesis conditions were screened by staining LDs with BODIPY 493/503 (5 μM, MedChemExpress, USA) and counterstaining nuclei with DAPI (7 μM, Beyotime, China).

    Techniques: Immunofluorescence, Fluorescence, Staining, Western Blot, Biomarker Discovery, Expressing

    Reagents details.

    Journal: Stem cell research

    Article Title: Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the PSEN1 H163R mutation

    doi: 10.1016/j.scr.2024.103495

    Figure Lengend Snippet: Reagents details.

    Article Snippet: Nuclei counterstain , DAPI (D1306) , 200 ng/μL , Invitrogen Cat#D1306, RRID: AB_2629482.

    Techniques: Immunocytochemistry, Control, Electroporation, Mutagenesis, Sequencing, Modification